By means of data from many spines The authors found that ARPC-2, a part of the Arp2 / 3 protein complex, which is concentrated in a ring at a fixed distance from the plasma membrane, about midway between the back and neck of the postsynaptic density. This area was formerly defined as endocytic zone, rich in proteins involved in activity-dependent endocytosis and receptor trafficking. This is consistent with earlier evidence , Arp2 / 3 involved in clathrin – coated vesicle formation and movement. The localization also suggests that Arp2 / 3. As a potential mediator of synaptic plasticity.. This Week localization in spines, Frizzled role in neuron survival, Horizontal Cell Feedback To Rods, MARK2 kinase in migrationBranching actin protein localization in spines Bence R cz and Richard J.
Sapir et al. Used in utero electroporation of short hairpin RNAs targeting to introduce MARK2 and shRNA – resistant cDNA MARK2 in developing mouse cortex. After MARK2 knockdown failed neurons to migrate through the intermediate zone , and them at a them at a normal bipolar morphology. MARK2 elevating levels also blocked the migration from the central zone, which indicates microtubule must come within a specified range for migration. The impact of the knock-down was MARK2 might due to malfunction of microtubule dynamics, because increased staining for stabilized microtubules, protein turnover decreased at the tips of microtubule polymerization and slowed. Continue reading